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The homogenate was filtered through a 100-m cell strainer and centrifuged at 500 g , followed by transfer of the floated adipocytes to an Eppendorf tube for RNA isolation

Peptides were injected onto a C18 column (75 m inner diameter 50 cm double nanoViper PepMap Neo, 2 m, 100 , Thermo Scientific) regulated at 50 C and separated with a linear gradient from 100% buffer A (100% H 2 O and 0.1% formic acid) to 28% buffer B (100% acetonitrile and 0.1% formic acid) at a flow rate of 300 nl min 1 over 104 min

Some studies have shown that sulforaphane blocks mutations in DNA that lead to cancer

FOXO4 transcriptional activity is regulated by monoubiquitination and USP7/HAUSP

Early reductions in glutamine levels are associated with increased oxidative stress and poor clinical outcomes

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