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Ni-NTA agarose beads (30210, Qiagen) were washed three times with NH 4 HCO 3 buffer (50 mM NH 4 HCO 3 , pH 7.4)

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f , ABTS antioxidant assay (see Methods) for the proportion of ABTS radical quenched by supernatant isolated from the indicated strains after 72 h incubation in l-asparagine minimal medium (lacking l-DOPA)