Experimental Consistency: Using the same sterile solvent across multiple protein preparations improves reproducibility and reduces unwanted microbial variables
By tissue type, the application that has the strongest evidence base is soft tissue: muscle strains, ligament sprains, and chronic tendinopathies
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Common reasons for deficiency include: Low intake particularly diets low in whole grains, legumes, leafy greens or animal products Gut health issues like low stomach acid, SIBO, coeliac disease, IBD, or other factors affecting absorption Medications such as the contraceptive pill, metformin or antacids, which can deplete B vitamins Chronic stress increases demand for certain b vitamins, including B5 and B6 Vegan or vegetarian diets higher risk of B12 deficiency, which can be corrected through supplementation Genetic reasons some mutations on certain genes can increase a persons need for B vitamins This is why I always recommend testing before supplementing
Dosage and Protocol Duration: Dosage in preclinical studies is often calculated based on body weight, typically in micrograms (mcg) per kilogram (kg)
Follow trends, not daily ups and downs